A British Airways plane from Budapest sparked a security alert as an ambulance "hazardous area" team rushed to Heathrow Airport and passengers were "checked over".
The BA876 flight from the Hungarian capital landed shortly before 7pm.
A spokesman for Heathrow Airport told MyLondon: "Passengers were checked over by the ambulance service because of an incident on the plane."
They also confirmed that police, ambulance and fire services attended as is protocol with emergencies on flights.
London Fire Brigade and the London Ambulance Service confirmed they attended.
A spokesman for London Ambulance Service said: "We were called at 6.55pm today (November 7) to reports of an incident at Heathrow Airport, Hounslow.
“We dispatched an ambulance crew, two incident response officers and our hazardous area response team (HART) to the scene.
“We assessed five people at the scene but did not take anyone to hospital.”
A British Airways spokesman said: "Our aircraft requested a priority landing as a precaution after experiencing a minor technical issue.
"Our teams looked after our customers and helped get them on their way as soon as possible.
"We are sorry to our customers for the delay to their travel plans."
Geraldine Fusciardi, who was one of the passengers detained, told MyLondon: "We were locked in the room for about 40 minutes.
"One woman collapsed in distress just as they were letting us out. No apology.
"Five minutes after they told us it was a major incident and physically prevented people from leaving the room – including some distressed women who needed the toilet – they released us all with some vague comments about engineering issue on plane."
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